妊娠糖尿病患者肠道菌群和炎症因子的相关性

摘要

Objective To detect the number of Bifidobacterium and Lactobacillus and the correlation with inflam-matory factors in gestational diabetes mellitus(GDM).Methods Collect healthy pregnant women and gestational diabe-tes patient's blood and feces.Using automatic biochemical analyzer analyzed clinical biochemical indicators.The number of Bifidobacterium and Lactobacillus detected by qPCR.The expression of TNF-αand IL-6 detected by ELISA.Results In the study,we found that,compared to NGT group,the expression of TG,TC,LDL,HDL and FBG in the GDM group were significant increased (P < 0.05),the number of Bifidobacterium and Lactobacillus were significant dis-creased (P <0.05)and the expression of CRP,TNF-αand IL-6 were significant increased (P <0.05).Furthermore,we found that the number of Bifidobacterium and Lactobacillus were negative correlation with the expression of FBG, TNF-αand IL-6,but the expression of CRP had no correlation with the number of Bifidobacterium and Lactobacillus. Conclusion The number of Bifidobacterium and Lactobacillus were discreased in GDM presumed that the Bifidobacte-rium and Lactobacillus play important role in the development of GDM,which was correlation with the expression of TNF-αand IL-6.%目的：检测妊娠糖尿病患者粪便中双歧杆菌和乳酸杆菌的变化，分析其和炎症因子的相关性。方法收集健康妊娠妇女以及妊娠糖尿病患者的血液以及粪便，采用全自动生化分析仪检测临床生化指标，荧光定量 PCR 检测肠道菌群双歧杆菌和乳酸杆菌的数量，ELISA 检测血液中炎症因子 TNF-α和 IL-6的含量。结果研究表明妊娠糖尿病患者组（GDM 组）的 TG、TC、LDL、HDL 以及 FPG 均高于 NGT 组，差异显著具有统计学意义（P ＜0．05）。与NGT 组相比，GDM 组肠道双歧杆菌以及乳酸杆菌的数量均减少，差异显著具有统计学意义（均 P ＜0．05），GDM 患者组 CRP、TNF-α以及 IL-6的含量均增加，差异显著具有统计学意义（均 P ＜0．05）。进一步研究发现双歧杆菌、乳酸杆菌和炎症因子、空腹血糖浓度具有密切的负相关性，和 CRP 浓度没有相关性。结论GDM 患者双歧杆菌和乳酸杆菌总菌数量减少，推测双歧杆菌和乳酸杆菌总菌在 GDM 的发生发展中存在一定作用，该作用可能和降低炎症因子TNF-α以及 IL-6的表达有关。