目的：通过11β-类固醇脱氢酶-2（11β-hydroxysteroid dehydrogenase-2，11β-HSD-2）基因启动子区甲基化发生率的测定，探讨原发性高血压可能的发病机制。方法选取未经治疗的原发性高血压患者64例以及正常对照组30例，Sequenom MassArray 质谱法检测11β-HSD-2基因启动子区“-101到461”的563个碱基序列的甲基化发生率。结果原发性高血压组甲基化发生率高且差异有统计学意义，CpG 位点如下：CpG＿7、8、CpG＿9、CpG＿62、63、64、CpG＿65、CpG＿72。t 值分别为－5．42，－3．17，－2．84，－2．32和－2．32，P ＜0．05。CpG＿7、8、CpG＿9位于核心启动子区。结论原发性高血压患者11β-HSD-2基因启动子区存在高甲基化发生率位点。%Objective By detecting the methylation rate of 11β-hydroxysteroid dehydrogenase-2 promoter in patients suffering from primary hypertension,to investigate the mechanism of primary hypertension.Methods To select 64 ca-ses of untreated patients suffering from primary hypertension and 30 healthy persons as controls.The CpG islands of 11β-HSD-2 gene promoter include 563 bases from “-101 to 461”.To detect its methylation rate using Sequenom Mas-sArray method.Results The methylation rates are higher at CpG_7、8、CpG_9、CpG_62、63、64、CpG_65 and CpG_72 in patients suffering from primary hypertension,and the differences are significant,t = - 5.42,- 3.17,- 2.84,- 2.32 and -2.32,P <0.05.CpG_7、8 and CpG_9 are in the core promoter sequence.Conclusion Patients suffering from pri-mary hypertension have hypermethylation frequency sites in the promoter region of 11β-hydroxysteroid dehydrogenase-2 gene as compared to the control group.
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