Modifying steroid in yeast, useful especially for preparing 11-deoxycortisol, by treatment with yeast having reduced 20alpha-hydroxysteroid dehydrogenase activity

摘要

Described are yeast strains, methods and genetic constructs for preparing them, and their use for synthesising or modifying steroid compounds. More particularly, strains having reduced 20á-HSD activity, in particular by modification of the GCY1 and/or YPR1 genes. The east strains described enable enhancement of the efficacy of synthesis and increase selectivity of yields of the process, as well as the quality of the final product. The yeast strains, methods and compounds are useful for researching, developing and producing products with therapeutic or prophylactic activity, in steroid derivatives for a human or an animal.