saccharomyces cerevisiae strains with modified ypr1 and gcy1 genes and having reduced 20 alpha-hydroxysteroid dehydrogenase (20 alpha-hsd) activity, their use in the preparation of steroidal compounds, as well as modification processes of hydroxyprogesterone and 11-deoxycortisol

摘要

"Modified yeasts and uses, mainly for the production of steroid derivatives". The present invention relates to novel yeast strains, processes and genetic constructs for their preparation, as well as their use for the synthesis or modification of steroid compounds. more particularly, the invention discloses strains that exhibit decreased 20Î ± -hsd type activity, notably by modification of the gcy1 and / or ypr1 genes. The yeast strains of the invention make it possible to improve synthesis efficiency or increase process selectivity or yields as well as the quality of the final product. The strains, processes and compounds of the invention are useful in the research, development and production of products with therapeutic or prophylactic activity in man or animal, notably steroid derivatives.