Objective:To investigate the effect of Rhizoma Nelumbinis on expression of p-JAK2,p-STAT3,Bcl-2 and Bax in renal tissues of diabetic nephropathy rats. Methods:60 SD male rats were randomly divided into 6 groups:the normal control group(group N),diabetic nephropathic model group(group DN),low-dose Rhizoma Nelumbinis group (RL,1. 5 g·kg-1·d-1), medium-dose Rhizoma Nelumbinis group(RM,3. 0 g·kg-1·d-1),high-dose Rhizoma Nelumbinis group(RH,6. 0 g·kg-1· d-1 ) and losartan potassium group ( LP, 30 mg · kg-1 · d-1 ) . Rats were treated respectively by intragastric administration for 12 weeks. Meanwhile rats of group N and group DN received the same volume distilled water by gavage. Blood serum creatinine,blood urea nitrogen and 24 h urinary protein were measured after the research. The renal pathological changes were observed by histochemis-try staining and electron microscope. The expression of p-JAK2,p-STAT3,Bcl-2 and Bax were detected by immunohistochemis-try. The cell apoptosis was detected by TUNEL. Results:Compared with group N,the glomerular became hypertrophic and the mesang-ial matrix increased in group DN. BUN,Scr,24 h urinary protein also increased significantly (P<0. 05). Meanwhile the expression of Bax,p-JAK2,p-STAT3 in renal tissue increased,the expression of Bcl-2 decreased significantly(P<0. 05). Compared with group DN,group RM and group RH had significantly lower 24 h urinary protein (P<0. 05),the pathology changes were relieved,cell apop-tosis was reduced,and the expression of Bax,p-JAK2,p-STAT3 decreased and the expression of Bcl-2 increased (P<0. 05). In addition,the effect of reducing urinary protein in group RM,RH was less than those of group LP. Conclusion:Rhizoma Nelumbinis may reducing urinary protein and delay the injury in diabetic nephropathy by down-regulating the level of Bax,p-JAK2,p-STAT3, up-regulating the level of Bcl-2.%也页目的：探讨藕节对糖尿病肾病大鼠肾组织p-JAK2、p-STAT3及凋亡因子Bcl-2、Bax表达的影响。方法：健康雄性SD大鼠60只,随机选取10只为正常组( N组),其余采用单次腹腔注射链尿佐菌素( STZ,45 mg/kg)制作DN模型,造模成功后随机分为糖尿病肾病组(DN)、藕节小剂量组(RL,1.5 g·kg-1·d-1)、中剂量组(RM,3.0 g·kg-1·d-1)、大剂量组(RH,6.0 g·kg-1·d-1)及氯沙坦钾组(LP,30 mg·kg-1·d-1),均采用灌胃给药,N组和DN组给予等量蒸馏水。12周后检测大鼠生化指标；HE、Masson染色及电镜观察肾脏病理改变；免疫组化法测定p-JAK2、p-STAT3、Bcl-2及Bax在肾组织表达情况；原位末端标记法( TUNEL)检测肾组织细胞凋亡情况。结果：实验12周末,与N组比较,DN组大鼠肾小球肥大、系膜基质增多、细胞凋亡明显,BUN、Scr、24 h尿蛋白定量明显升高(P<0.05),肾组织Bax、p-JAK2、p-STAT3表达明显上调,Bcl-2表达下调(P<0.05)；与DN组比较,藕节中、高剂量组肾脏病理改变减轻、细胞凋亡减少,24 h尿蛋白定量较DN组明显降低(P<0.05),但降尿蛋白作用弱于氯沙坦钾组,同时肾组织Bax、p-JAK2、p-STAT3表达下调,Bcl-2表达上调(P<0.05)。结论：藕节可能通过上调Bcl-2在肾组织的表达,下调Bax、p-JAK2、p-STAT3的表达,从而减少尿蛋白,延缓DN进展。
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