Objective:To observe the effect of Huayu Tongluo traditional Chinese medicine on metabolic parameters and nephrin expression in diabetic nephropathy rats,and to explore the possible mechanism of Huayu Tongluo traditional Chinese medicine in preventing and treating DN. Methods:The 40 rats were randomly selected as the normal control group(n=10),the other 30 rats were injected with 1% streptozotocin after feeding high-fat and high-glucose diet for one month. The diabetic model rats( blood glucose≥16. 7 mmol/L)were randomly divided into the model group( M group),HYTL group( Z group),the irbesartan group ( I group). The Z and I groups were given corresponding drugs. 24 h urine protein was tested on the third day and at the end of the 4 th,8 th,16 th week. At the end of the 16th week. the blood creatinine(Cr)、urea nitrogen(BUN)and uric acid(UA)of the rats were detected and the renal pathological changes were observed by light microscope. The expressions of nephrin in renal cortex were detected by immunohistochemistry staining and reverse transcription polymerase chain reaction( RT-PCR). Results:Compared with the M group,at the 16th weekend,24 hour urinary protein,blood creatinine in Z group were obviously improved significantly( P﹤0. 05). Compared with the I group,at the same time,at the end of the 8th week,24 h urine protein quantitation in z groups elevated significantly(P﹤0. 05),at the end of the 16th week,24 h urine protein quantitation in z groups reduced significantly(P﹤0. 05). At the 16th weekend,by Semi quantitative analysis,the expression of nephrin in the Z group was significantly higher than in the M group(P﹤0. 05). The expression of nephrin mRNA in the Z group was significantly higher than in the M group(P﹤0. 05). There was no significant difference between the two treatment groups(P﹥0. 05). Conclusion:Huayu tongluo traditional Chinese medicine can protect renal function and reduce proteinuria. Its mechanism may be related to the up-regulating mRNA expression of nephrin.%目的：观察化瘀通络中药对糖尿病肾病（ DN）大鼠代谢指标和裂孔膜蛋白nephrin表达的影响，探讨化瘀通络中药防治DN的可能作用机制。方法：将40只大鼠随机分为空白对照组10只、造模组30只，造模组高糖高脂饲料喂养1个月，采用一次性腹腔注射链尿佐菌素的方法复制糖尿病模型，成模大鼠随机分为中药组、西药对照组（厄贝沙坦组）、模型组，中药组和厄贝沙坦组分别给予相应药物，各组于第3天、4周、8周、16周测24 h尿蛋白，16周末处死大鼠，全自动生化分析仪检测血肌酐、尿素氮、尿酸。光镜观察肾脏病理改变情况，采用免疫组织化学法检测nephrin的表达并进行半定量分析， RT-PCR法检测nephrin mRNA表达情况。结果：与模型组相比，第16周末中药组24 h尿蛋白、肌酐等各项指标均有明显改善，差异具有统计学意义（ P﹤0．05）；与厄贝沙坦组同时间点比较，第8周末中药组24 h尿蛋白明显升高（ P﹤0．05），第16周末中药组24 h尿蛋白降低（P﹤0．05）；第16周末nephrin半定量分析结果显示中药组较模型组表达明显增加（P﹤0．05）；中药组nephrin mRNA的表达较模型组明显增强（ P﹤0．05），两治疗组之间该项指标差异无统计学意义（ P﹥0．05）。结论：化瘀通络中药可以减少蛋白尿，保护肾功能的作用可能与上调足细胞裂孔膜蛋白nephrin的表达有关。
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