Objective To develop a method for detetmining mesaconitine and aconitine in Yaotong Mixture. Methods RP-HPLC was used for the determination of mesaconitine and aconitine. The column was Sunfire C18 (4.6 mm 250 mm, 5 μm). The mobile phase was acetonitrile-0.2% acetic acid (adjusted pH value at 6.30 by triethylamine), and the gradient elution was used. The detection wavelength was set at 232 nm, and the flow rate was 1.0 mL/min. Results Mesaconitine and aconitine showed a good linear relationship in the range of 0.007 2－0.072 μg (r＝0.999 5) and 0.004 6－0. 046 μg (r＝0.999 8) respectively. The average recovery of mesaconitine was 98.2% with RSD of 1.6% (n＝6), and the average recovery of aconitine was 97.4 % with RSD of 1.9% (n＝6). Conclusion The method is accurate, simple and fast, and can be used for the determination of mesacontine and aconitine in Yaotong Mixture.%目的 建立腰痛合剂中次乌头碱和乌头碱的含量测定方法.方法 采用反相高效液相色谱法测定腰痛合剂中次乌头碱和乌头碱含量,色谱柱SunFire C18(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%冰乙酸(用三乙胺调pH为6.30),梯度洗脱,流速1 mL/min,检测波长232 nm,柱温为30 ℃.结果 次乌头碱和乌头碱分别在进样量0.007 2～0.072 μg、0.004 6～0.046 μg范围内与峰面积呈良好的线性关系,r分别为0.999 5、0.999 8,平均加样回收率分别为98.2% (RSD＝1.6%)、97.4%(RSD＝1.9%).结论 该方法准确、方便、快速,可以用来测定腰痛合剂中次乌头碱和乌头碱的含量.
展开▼
