HPLC同时测定紫朱软膏中左旋紫草素和β,β'-二甲基丙烯酰阿卡宁含量

摘要

目的 建立同时测定紫朱软膏中紫草的左旋紫草素和β,β'-二甲基丙烯酰阿卡宁含量的高效液相色谱方法.方法 采用Welch Materials XB-C18色谱柱(4.6 mm×250 mm,18.5μm),流动相乙腈-水-甲酸(75:25:0.05),检测波长516 nm,柱温30 ℃,流速1.0 mL/min,进样量20μL,对紫朱软膏中紫草的有效成分左旋紫草素和β,β'-二甲基丙烯酰阿卡宁进行定量测定.结果 左旋紫草素在0.025 6~0.256μg范围内线性关系良好(r2=0.999 5),平均回收率为98.12%(RSD=1.92%);β,β'-二甲基丙烯酰阿卡宁在0.174 4~2.616μg范围内线性关系良好(r2=0.999 2),平均回收率为100.46%(RSD=1.17%).结论 本试验建立的方法简便,测定结果准确,可用于紫朱软膏的质量控制和评价.%Objective To establish an HPLC method to simultaneously determine the contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment.Methods The contents of shikonin andβ,β'-dimethylacrylshikonin inZizhu Ointment were determined by HPLC. The chromatographic conditions were Welch Materials XB-C18 Column (4.6 mm × 250 mm, 18.5μm), with mobile phase of acetonitrile-water-formic acid (75:25:0.05), detection wavelength of 516 nm, temperature of 30℃, flow rate of 1.0 mL/min and the load of 20μL.Results Shikonin showed a good linearity in the range of 0.025 6-0.256μg (r2=0.999 5), and the average recovery rate was 98.12% (RSD=1.92%).β,β'-dimethylacrylshikonin showed a good linearity in the range of 0.174 4-2.616μg (r2=0.999 2), and the average recovery rate was 100.46% (RSD=1.17%).Conclusion This method is simple and accurate, which can be used for the quality control and evaluation of Zizhu Ointment.