糖网明目颗粒不同提取部位对缺氧诱导的血管内皮细胞中相关基因表达的影响

摘要

ObjectiveTo observe the effect of different extracts ofTangwang Mingmu Granule on hypoxia induced gene expressions in vascular endothelial cells.Methods COCl2 intervention cells were used to copy hypoxia models. Human umbilical vein endothelial cells EA. Hy926 were divided into blank group, hypoxia model group,Tangwang Mingmu Granule group, extract 1 (glycosides and flavonoids) group, extract 2 (orgain acids and polysaccharides) group and extract 3 (alkaloids) group. The changes in gene expressions of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α mRNA were detected by semi-quantitative RT-PCR.ResultsThe gene expression levels of VEGF, VEGFR-1, VEGFR-2, ICAM-1 and IL-1α were significantly up-regulated under the hypoxic condition (P<0.05), and the ratio of VEGFR-1/VEGFR-2 was significantly reduced. However,Tangwang Mingmu Granule significantly reversed the expressions of these genes.Conclusion The function intensity of gene expressions weakens in the following sequence:glycosides and flavonoids>alkaloids>organic acids and polysaccharides inTangwang Mingmu Granule.%目的：探讨糖网明目颗粒不同提取部位对缺氧诱导的人脐静脉内皮细胞EA.hy926中相关基因表达的作用机制。方法以COCl2干预细胞复制缺氧模型,人脐静脉内皮细胞EA.hy926分为空白组、缺氧模型组、全方组、部位1组(苷类和黄酮类)、部位2组(有机酸和多糖类)和部位3组(生物碱类)。采用半定量RT-PCR检测糖网明目颗粒不同提取部位对缺氧诱导细胞中血管内皮细胞生长因子(VEGF)及其受体(VEGFR)1、2和细胞间黏附分子(ICAM)-1、白细胞介素(IL)-1α基因表达的变化。结果缺氧导致EA.hy926细胞VEGF、VEGFR-1、VEGFR-2、ICAM-1、IL-1α基因表达上调(P＜0.05),VEGFR-1/VEGFR-2比值降低。糖网明目颗粒及其不同提取部位干预细胞后,VEGF、VEGFR-1、VEGFR-2、ICAM-1、IL-1α基因表达降低(P＜0.05), VEGFR-1/VEGFR-2比值升高。结论糖网明目颗粒中苷类和黄酮类、生物碱类及有机酸和多糖类调控缺氧诱导的血管内皮细胞基因表达的作用强度依次减弱。