糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞肿瘤坏死因子-α、白细胞介素-1α表达的影响

摘要

ObjectiveTo observe the effects ofTangwang Mingmu Granules on inflammation- associated cytokines in vascular endothelial cells EA.hy926 under hypoxic/high glucose. MethodsCoCl2 and high glucose were used to make the hypoxic and high glucose model in human umbilical vein endothelial cell line EA.hy926. EA.hy926 cell proliferation was measured by MTT method. Gene and protein expression levels of inflammatory factors were detected by semi-quantitative RT-PCR and ELISA assay.ResultsBoth hypoxia and high glucose promoted EA.hy926 cell proliferation. The gene and protein expressions of TNF-α and IL-1α were significantly up-regulated under the same condition (P<0.05). The cell proliferation, TNF-α, IL-1α mRNA and protein expression levels were significantly reduced by treatment of Tangwang Mingmu Granules (P<0.05).Conclusion The preventive and therapeutic effect ofTangwang Mingmu Granules on diabetic retinopathy might be related to down-regulate the expression of inflammation-associated cytokines of mRNA and protein, such as TNF-α and IL-1α.%目的 观察糖网明目颗粒提取物对缺氧/高糖状态下血管内皮细胞EA.hy926炎症相关因子的影响,并探讨其作用机制.方法 以CoCl2和高浓度葡萄糖干预人脐静脉内皮细胞EA.hy926复制缺氧和高糖模型,MTT法检测细胞增殖能力,半定量RT-PCR检测炎症相关基因表达,ELISA检测炎症相关蛋白表达.结果 缺氧和高糖均能促进EA.hy926细胞增殖,显著上调肿瘤坏死因子-α(TNF-α)、白细胞介素-1α(IL-1α)基因和蛋白表达(P<0.05).药物干预后,细胞增殖能力降低,TNF-α、IL-1α基因和蛋白表达水平显著降低(P<0.05).结论 糖网明目颗粒提取物可能通过调控缺氧/高糖状态下内皮细胞 TNF-α、IL-1α等炎症相关因子的 mRNA和蛋白表达从而达到防治糖尿病视网膜病变的目的.