Graves病患者131I治疗前后CD4+CD25+Foxp3+调节性T细胞的变化

摘要

Objective:To explore whether I reverted the immune disorder of Graves' disease (GD) via changing the proportion of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) , we evaluated the peripheral lymphocyte subsets ,Tregs and the expression of Foxp3 mRNA from GD patients before I administration and in the first month after I administration. Methods: Thirty GD patients treated by I were included in this study. The proportion of lymphocyte subsets in the peripheral blood from GD before I administration and in the first month after I administration were detected by flow cytometric analysis . Real-time polymerase chain reaction was performed also to measure the expression of Foxp3mRNA. Results:Compared to GD patients before I administration, the proportion of CD3 CD19+ B and CD3 CD4 T lymphocytes was significantly lower (P <0. 05) , and the proportion of CD3 CD16 CD56 NK lymphocytes was significantly higher in GD patients in the first month after I administration ( P < 0. 05 ) , the proportion of CD3 CD8 T lymphocytes, Tregs and the expression of Foxp 3mRNA could not be changed remarkably. Conclusion: Iodine431 might not alleviate impairment of immunological tolerance via increasing the proportion of Tregs , while it might control excessive immunological response via decreasing the proportion of CD3- CD19 + B and CD3 + CD4 + T lymphocytes and increasing the proportion of NK lymphocytes .%目的:通过对Graves病患者131I治疗前后外周血各淋巴细胞亚群和CD4+CD25+Foxp3+调节性T细胞含量以及相关基因Foxp3mRNA表达水平的测定,探讨131I治疗方法是否可以通过改变调节性T细胞的含量从而改善Graves病患者的免疫功能异常.方法:采集30例Graves病患者131I治疗前及治疗后1个月的外周血,流式细胞仪检测外周血CD3+、CD3+CD4+、CD3+CD8+、CD3-CD19+、CD3- CD16+CD56+淋巴细胞亚群及CD4+CD25+Foxp3+ 调节性T细胞的百分率,Real-Time PCR检测外周血单个核细胞Foxp3mRNA的表达水平.结果:和治疗前相比,Graves病患者131I治疗1个月后外周血CD3+CD4+T细胞和CD3-CD19+B细胞百分率都明显减少(P <0.05),CD3-CD16+CD56+NK细胞百分率明显增加(P <0.05),CD3-CD8+T细胞、CD4+CD25+Foxp3+调节性T细胞百分率和Foxp3mRNA的表达水平无明显变化.结论:131I 不能通过增加调节性T细胞的含量而改善Graves病患者的免疫耐受障碍,但可以通过减少CD4+T和B淋巴细胞的含量和增加NK细胞的含量来控制过度的免疫应答.