四种中药单体作为肿瘤细胞疫苗佐剂的体外筛选研究

摘要

目的：初步从细胞水平检测原花青素、天麻素、黄芩素、芹菜素四种中药单体对抗原提呈细胞RAW264.7和DC2.4的免疫刺激活性，为肿瘤细胞疫苗佐剂筛选和应用提供理论基础。方法：以LPS作为阳性对照，不同种类、不同浓度的中药单体分别刺激RAW264.7和DC2.4细胞系，48 h后用抗体染色，通过流式细胞仪检测两种细胞表面CD80、CD86、MHCⅠ、MHCⅡ分子的表达量。结果：实验结果显示不同种类、不同浓度的中药单体分别刺激RAW264.7和DC2.4细胞后，细胞表面CD80、CD86、MHCⅠ、MHCⅡ分子不同程度表达上调，原花青素和黄芩素在细胞水平具有较强的免疫刺激活性。结论：原花青素、黄芩素是两种具有潜在应用价值的肿瘤细胞疫苗佐剂。%Objective:To detect immunological effects of procyanidin ,gastrodin,baicalein and apigenin on antigen presenting cells of RAW264.7 and DC2.4 and search for new tumor vaccine adjuvant.Methods: After different concentrations of four kinds of Chinese herbal monomer and LPS were used to stimulate RAW 264.7 or DC2.4 cells for 48 hours,cells were obtained and stained with CD80,CD86,MHCⅠand MHCⅡ antibody.Then protein expression levels of CD80,CD86,MHCⅠ and MHCⅡ on RAW264.7 and DC2.4 cells were analyzed by flow cytometry.Results: Results showed that after 48 hours stimulated with different concentrations of four kinds of Chinese herbal monomer on RAW 264.7 or DC2.4 cells,the expression levels of CD80,CD86,MHCⅠ,MHCⅡ were all up-regulated in comparison with control , procyanidin and baicalein exhibited stronger immunological stimulating activity on a cellular level.Conclusion:Procyanidin and baicalein were found having potential application value in clinical treatment as tumor vaccine adju -vants.