不同分型卵巢癌患者组织中瘦素mRNA表达的相关研究

摘要

Objective:To explore the mRNA differential expression and clinical effect of leptin in different kinds of ovarian cancer.Methods: 129 patients with ovarian cancer and 42 controls were collected from Jun.2015 to Jun.2016 in our hospital.We got a small tissue by ovarian biopsy guided under the ultrasonic processing,detected the total RNA,purified mRNA and analyzed the correlation of the expression of leptin in different kinds and clinical stages of ovarian cancer.Results: Compared with the control groups,the mRNA expression of leptin in each group was increased(P<0.05),and the serous adenocarcinoma group increased obviously(P<0.05).Patients with clinical stage FIGO Ⅲ was significantly higher than other stages in serous adenocarcinoma group(P<0.05).Conclusion: Leptin could express in different kinds and clinical stages of ovarian cancer,and may be a new detection index for early diagnosis of ovarian cancer.%目的:探讨不同分型卵巢癌患者组织中瘦素(Leptin)mRNA的表达差异及临床价值.方法:收集我院2015年6月~2016年6月收治的卵巢癌患者129例及正常对照42例,于超声引导下行卵巢穿刺活检术,取少量卵巢周围组织,提取总RNA并纯化mRNA,运用RT-PCR技术检测各组leptin mRNA的表达情况,并分析leptin表达与不同分期,不同病理分型相关性.结果:与正常对照组相比,各组卵巢组织中leptin mRNA的表达均有所升高(P<0.05),且浆液性腺癌组leptin mRNA的表达尤为明显(P<0.05);浆液性腺癌组各临床分期中,Ⅲ期卵巢组织中leptin mRNA的表达最为显著(P<0.05).结论:Leptin在不同分型不同分期卵巢癌组织中有一定表达差异,可能成为一种早期检测、诊断卵巢癌的新型指标.