肝癌患者手术前后血管内皮细胞相关细胞因子的变化

摘要

目的 检测肝细胞癌行根治性手术切除前后m管内皮细胞生长相关细胞因子的变化.方法 对照组1:健康人群30例;对照组2:肝血管瘤手术切除患者30例;实验组1:肝硬化患者30例;实验组2:肝癌根治性手术切除患者30例.利用生物蛋白芯片技术检测血清中细胞因子含量.结果 肝癌患者血清中细胞因子IL-1β、IL-6、IL-8、血管内皮生长因子(VEGF)和表皮生长因子(EGF)含量明显高于健康对照组和肝硬化组(P<0.01);细胞因子IL-10、γ干扰素(IFNT)和α肿瘤坏死因子(TNFα)含量明显低于健康对照组和肝硬化组(P<0.01).肝癌患者根治性手术后,血清中细胞因子IL-1β、IL-6、IL-8、VEGF和EGF的含量明显低于术前(P<0.01);而细胞因子IL-10、IFNγ和TNFα的含量明显高于术前(P<0.01).术后血清中细胞因子含量的总体水平与肝硬化患者接近(P>0.05).结论 肝细胞癌患者血清中促进血管内皮细胞生长的细胞因子的含量明显升高;而抑制血管内皮细胞生长的细胞因子的含量明显降低.根治性手术后肝细胞癌患者血清中促进血管内皮细胞生长的细胞因子的含量明显下降;而抑制血管内皮细胞生长的细胞因子的含量明显升高.%Objective To determine the serum levels of cytokine IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, IFNγ, EGF, MCP-1 and TNFα in preoperative and postoperative patients with hepatocarcinoma(HCC). Methods 30 patients with hepatocirrhosis were taken as hepatocirrho-sis group; 30 normal health examiners were taken as the normal control group. 30 patients with hepatocarcinoma were taken as HCC group; 30 patients with hepatic hemangioma were taken as the control group. Cytokines had been measured by biochips methods in evidence 180 automatic biochips analyze.Results The serum levels of IL-1β, IL-6, IL-8, VEGF and EGF of the patient with HCC and cirrhosis were significantly higher than those in normal control group. The serum levels of IL-10, IFNγand TNFα of the patient with HCC and cirrhosis were significantly lower than those in normal control group. The serum levels of IL-1β, IL-6, IL-8, VEGF and EGF of the preoperative patient with HCC were significantly higher than those in the postoperative patient with HCC. The serum levels of IL-10,IFNγ and TNFa of the preoperative patient with HCC were significantly lower than those in the postoperative patient with HCC. Conclusions These results suggest that the serum levels of angiogenic factors in HCC were increased. The serum levels of the inhibitors of vascular endothelial cells in HCC were decreased. The serum levels of angiogenic factors in the postoperative patient with HCC were decreased. The serum levels of inhibitors of vascular endothelial cells in the postoperative patient with HCC were increased.