3T3-L1前脂肪细胞分化过程中chemerin基因表达水平的变化

摘要

Objective To investigate the change of chemerin gene expression of 3T3-L1 preadipocytes during the process of differentiation in vitro,and to explore the relationship between chemerin gene and adipocytes differentiation. Methods 3T3-L1 preadipocytes were induced to mature adipocytes with l-methyl-3-isobutylxanthine, dexamethasone and insulin. Cell differentiation and lipid accumulation were determined by Oil Red 0 staining. The expression of chemerin in 3T3-L1 adipocytes was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot Results During the 3T3-L1 adipocyte differentiation, the expression of chemerin mRNA was increased , and kept higher level and remained unchanged on the 6th day. The results of Western blot showed that the expression of chemerin protein was also increased. Conclusions The augmentation of chemerin mRNA and protein during differentiation suggests that it probably participate in adipocyte differentiation and lipid accumulation.%目的 探讨体外培养3T3-L1前脂肪细胞诱导分化过程中chemerin基因表达水平的变化与脂肪细胞分化、脂质积聚之间的关系.方法 应用3-异丁基-1-甲基黄嘌呤、胰岛紊、地塞米松联合方案诱导其分化为成熟的脂肪细胞,采用油红0染色观察脂肪细胞分化及脂质聚集情况,并应用RT-PCR和Western印迹技术检测chemerin基因表达的变化.结果 3T3 -L1脂肪细胞分化过程中,chemerin mRNA表达水平逐渐升高,分化至第6天达到较高水平且逐渐趋于稳定.利用Western印迹可观察到,随着脂肪细胞分化成熟.chemerin基因的蛋白表达水平逐渐增高.结论 chemerin mRNA及蛋白质在脂肪细胞分化成熟过程中表达水平升高,提示其很有可能参与了脂肪细胞分化和脂质聚集.