慢性乙型重型肝炎患者外周血单个核细胞RORγt和Foxp3 mRNA表达分析

摘要

ObjectiveTo observe the expression levels of retinoic acid related orphan receptor γt (RORγt) and a fork head helix transcription factor (Foxp3) in peripheral blood mononuclear cell (PBMC) of patients with chronic severe hepatitis B, and explore its effect on the occurrence and development of chronic severe hepatitis B.Method26 patients with chronic severe hepatitis B (severe group), 20 patients with chronic serious hepatitis B (serious group) and 20 healthy volunteers (control group) were chosen as the research objects in our hospital from January 2010 to June 2012. The venous blood was collected, the expression levels of RORγ t and Foxp3 mRNA in PBMC were detected using RT-PCR; the levels of interleukin -17 (IL-17) and transforming growth factor beta (TGF-β) in ulture supernatants of PBMC were detected by ELISA.Result Compared with control group, the mRNA expreesion levels of RORγ t and Foxp3 in serious group and severe group were higher (P<0.05), the levels of IL-17 and TGF-β in culture supernatant of PBMC increased significantly (P<0.05). Compared with serious group, the mRNA expression level of Foxp3 was lower while RORγt was higher (P<0.05) in severe group, the level of IL-17 increased while TGF-β decreased signiifcantly (P<0.05). There was a signiifcant relationship between RORγt mRNA and IL-17 and TGF-β(P<0.05), while there was no signiifcant relationship between Foxp3 mRNA and IL-17 and TGF-β (P>0.05). ConclusionThe mRNA expression levels changes of RORγt and Foxp3 are related to the development of severe chronic hepatitis B, and its imbalanced expression may be a reason leading Th17/Treg cells’ imbalance.%目的：观察慢性乙型重型肝炎患者外周血单个核细胞（PBMC）维A酸相关孤独受体γt（RORγt）和叉头状螺旋转录因子（Foxp3）mRNA表达水平，探讨其对慢性乙型重型肝炎发生发展的影响。方法选择2010年1月至2012年6月本院收治的26例慢性乙型重型肝炎患者（重型组）、20例慢性乙型肝炎重度患者（重度组）及同期本院体检中心20例健康志愿者（对照组）作为研究对象。采集三组研究对象静脉血，采用逆转录-聚合酶链式反应（RT-PCR）检测PBMC RORγt和Foxp3 mRNA表达水平，采用酶联免疫吸附测定（ELISA）检测患者PBMC培养上清液中白细胞介素-17（IL-17）和转化生长因子-β（TGF-β）水平。结果与对照组比较，重度组和重型组患者PBMC RORγt和Foxp3 mRNA表达水平较高（P＜0.05），PBMC培养上清液中IL-17和TGF-β水平显著升高（P＜0.05）。与重度组比较，重型组患者Foxp3 mRNA表达水平较低而RORγt mRNA表达水平较高（P＜0.05）。PBMC培养上清液中IL-17水平明显升高而TGF-β水平显著下降（P＜0.05）。RORγt mRNA表达水平与IL-17和TGF-β存在显著相关性（P＜0.05），Foxp3 mRNA表达水平与IL-17和TGF-β无相关性（P＞0.05）。结论 RORγt和Foxp3 mRNA表达水平变化与慢性乙型重型肝炎发生发展有关，其表达失衡可能是导致慢性乙型肝炎患者外周血辅助性T细胞17/调节性T细胞（Th17/Treg）失衡的原因之一。