体液法医学鉴定miRNA检测方法的建立与应用

摘要

Objective To establish a SYBR Green real-time PCR detection method with tissue-specific miRNAs and explore a novel approach for forensic body fluid identification. Methods The frequently reported 6 standard miRNAs were synthesized to establish a SYBR Green method, and verify with body fluid. The relative expression data for the 6 miRNAs were obtained using SYBR Green real-time PCR method in peripheral blood, menstrual blood, saliva and semen. Results The assays showed that miRNA205 permitted the unequivocal identification among different fluids. miRNA451 and miRNA144 could be used to distinguish blood from non-blood. Menstrual blood or peripheral blood could be identified through miRNA214. miRNA888 and miRNA891 was highly expressed in semen. Conclusion The results of this study indicate that miRNA SYBR Green profiling may provide a feasible and effective approach to body fluid identification for forensic casework.%目的 建立针对体液特异性miRNA的SYBR Green检测方法,用于体液鉴定,探究法医实践中体液鉴定新方法.方法 根据文献选出6条miRNA为靶点,以合成的标准miRNA作为模板进行体系构建,再对实际样本进行验证,检测6条miRNA在外周血、月经血、唾液、精液中的相对表达量. 结果 miRNA205在不同体液间表达差异不明显;miRNA451、miRNA144可明显的区分血与非血;miRNA214可鉴定月经血;miRNA888、miRNA891在精液中高表达. 结论 建立的针对miRNA的SYBR Green检测方法合理有效,可用于法医实践中体液鉴定.