miRNA Detecting method for controlling miRNA and its application of biomarker for colon cancer

摘要

The present invention relates to: 1) a first step of extracting miRNA from an unknown bio sample; 2) a second step of obtaining miRNA data according to the expression level of the sequenced miRNA from the miRNA extracted in the first step; 3) acquiring tumor miRNA data for each expression level using a data platform from a whole milk map (TGCA); 4) obtaining miRNA results (1) for each expression level by comparing the expression level-specific miRNA data obtained in the second step with the expression level-specific tumor miRNA data obtained in the third step; 5) a fifth step of sequencing the miRNA result (1) according to the expression level obtained in the fourth step from 5 folds or more, 2.5 folds or more to less than 2.5 folds from less than 5 folds; 6) obtaining a rearranged miRNA result (2) from the sequenced miRNA data of the third step only at the expression level of 5 fold or more; 7) obtaining a list of cancer suppressor genes from the cancer suppressor gene database, and comparing the obtained cancer suppressor gene list and the sorted miRNA results (2) to obtain cancer screening gene screening results from common results; 8) comparing the results of the reordered miRNA obtained in the sixth step and (2) the results of screening the cancer suppressor gene obtained in the seventh step to obtain a common result by comparing the cancer suppressor gene with a target prediction database; 9) detecting a regulated miRNA ID for controlling a tumor suppressor gene from the common result obtained in the eighth step; And a method for detecting a regulatory miRNA for controlling a tumor suppressor gene. Furthermore, the present invention can be applied to colon cancer biomarkers and therapeutic agents by detecting colon cancer-controlling miRNAs.