密码子优化增强轮状病毒VP6基因重组腺病毒的体液免疫和攻毒保护效果的研究

摘要

目的 以表达野生型RVVP6基因的重组病毒rvAdVP6为对照,在小鼠模型上观察基因优化重组腺病毒rvAdVP6(o)的体液免疫效果和攻毒保护效果.方法 6到8周雌性BALB/c小鼠随机分组,通过等量病毒(每次108TCID50/只)3次滴鼻免疫,检测3次免疫后的血清抗体;并用鼠轮状病毒进行攻毒实验,检测不同组的免疫保护效果.结果 三次免疫rvAdVP6(o)产生的抗VP6血清IgG抗体水平均明显高于rvAdVP6;鼠轮状病毒攻击后检测小鼠的排毒量,发现rvAdVP6(o)免疫组的RV排毒量明显少于rvAdVP6.结论 在等量重组腺病毒免疫的情况下,优化后的VP6重组腺病毒在体液免疫和攻毒保护方面,均优于优化前.%Objective To observe the serum immune responses and protection in mice model of the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization (rvAdVP6 (o)) in comparison with the wild type(rvAdVP6).Methods 6 -8 week female BALB/c mice were randomly grouped and immunized three times intranasally with 108 TCID50 rvAdVP6(o) and rvAdVP6,respectively,then detect the serum IgG level against rotavirus induced by rvAdVP6(o) and rvAdVP6.The amount of sheding viral antigens in feces was detectd after mice rotavirus was taked orally.Results The serum IgG level against rotavirus induced by rvAdVP6 (o) was higher than that of rvAdVP6 after three times of immunization.The immunized mice shed lower amount of viral antigens in feces as compared with the rvAdVP6.Conclusion The recombinant adenovirus which encode optimized human rotavirus VP6 proteins (rvAdVP6(o)) could induce stronger serum immune and protective responses against the challenge of the rotavirus than the wild type(rvAdVP6) at the same immunizing dosage.