甘肃省202株鼠疫耶尔森菌基因型分布及流行特征分析

摘要

目的 研究甘肃省鼠疫耶尔森菌(鼠疫菌)基因分型分布及其流行特征.方法 根据鼠疫菌基因组23个差异区段设计引物,采用PCR技术,分析甘肃省202株不同来源的鼠疫菌.结果 202株鼠疫菌共分为8个基因型,即1b、5、7、8、13、26、GS1型(新基因型)和GS2型(新基因型).自20世纪60年代起至今分离到1b、8和GS1基因型,而自1977年后再未分离到7、13、26基因型,在1995年后才分离到GS2、5基因型.结论 甘肃省1b、8和GS1基因型鼠疫菌自20世纪60年代起持续流行至今,7、13和26基因型菌已有40余年未分离到,而GS2和5基因型菌自20世纪90年代才开始流行.%Objective To study the genotype distributions and epidemiological characteristics of Yersinia pestis in Gansu province.Methods Primers were designed according to the confirmed 23 differential sections,to genotype the 202 Yersinia pestis DNA of Gansu province by PCR,and to analyze its distribution and epidemiological characteristics.Results Yersinia pestis in Gansu province could be divided into eight genotypes:1b,5,7,8,13,26,new genotype 1 (GS1) and new genotype 2 (GS2).They were distributed in various regions.1b,8 and GS1 genotypes of Yersinia pestis had been identified since 1960s but the 7,13 and 26 genotypes had not been isolated for more than 40 years while GS2 and 5 genotypes had been isolated since 1990s.Conclusion 1b,8 and GS1 genotypes of Yersinia pestis continued to be violently prevalent since 1960s but 7,13 and 26 genotypes had not been isolated for more than 40 years while GS2 and 5 genotypes had started to be popular since 1990s.