STZ诱导恒河猴糖尿病动物模型的建立

摘要

Objective To make a reproducible diabetes mellitus model in macaques by injecting streptozotocin (STZ). Methods we used STZ (30 mg/kg of body weight, intravenously, 3days) to five macaques. The dynamic changes of the glucose, C-peptide and insulin contents in plasma were measured for 12 months. The IVGTT were made at 2 and 3 month. Results Three of five monkeys became markedly hyperglycemic, 2 other monkeys received a second and third dose of STZ intravenously (30 mg/kg) after the first dose. Compared with the contents before injection, the glucose contents increased significantly while C-peptide and insulin contents reduced in plasma in macaques 1 week after streptozotocin injection(P <0. 01) ,Diabetic monkeys also had abnormal responses to an intravenous glucose tolerance test at 2 and 3month. Conclusion The results showed that low dose with 30 mg/kg STZ could lead to the significant changes in glucose, C-peptide and insulin content stably, indicating that macaques diabetes mellitus model was established successfully.%目的 复制稳定的链脲佐菌素诱导糖尿病恒河猴动物模型.方法 健康恒河猴5只,小剂量( 30mg/kg)多次静脉注射链脲佐菌素,分别在注射后2、3个月进行葡萄糖耐量实验并连续观察血糖、胰岛素、C肽的变化.连续观察12个月.结果随着时间推移,动物出现典型的糖尿病症状.3只动物的血糖静脉注射1次后持续10周稳定,另外2只分别进行了第2、3次注射.动物血糖在12个月内平稳上升、胰岛素、C肽分泌持续下降.2个月时葡萄糖耐量减低明显(P＜0.01).结论 小剂量多次注射STZ后恒河猴可出现持续、稳定的糖尿病表现,可作为相关研究的动物模型.