LIVER FIBROSIS INDUCING VECTOR AND METHOD FOR ESTABLISHING ANIMAL MODEL, USING SAME

摘要

The present invention relates to a liver fibrosis inducing vector and a method for establishing an animal model, using the same. According to the present invention, treatment with tamoxifen at a specific time point allows a Cas9-ERT2 protein complex to translocate from the cytosol into the nucleus. As such, the protein complex that has translocated into the nucleus can more elaborately modify a specific DNA in a desired part at a desired time point with the aid of guide RNA (gRNA). Particularly, application of tamoxifen to a Cas9-ERT2 complex in which a gRNA of the liver fibrosis regulator gene TIF1γ is fused to a hepatic stellate cell-specific promoter knocks out the TIF1γ gene to cause liver fibrosis. TIF1γ is a very important factor in the prevention and treatment of liver fibrosis or liver cirrhosis. Therefore, the present invention can be utilized for developing a technology for analysis of liver fibrosis disease and further is useful for clinical studies such as screening of candidate drugs for treatment of liver fibrosis, with the expectation of finding applications to identifying novel proteins that may be targets of new drug development.