Objective To comparatively evaluate the therapeutic effect of puerarin on bone mineral density of the mandible and femur in ovariectomized rats, and to investigate the association between TNF-a, CRP and E2. Methods 40 female 3-month old SD rats were divided into 4 groups randomly: sham operation group ( sham) , ovariectomized ( OVX) group, ovariectomized group with injection of estrogen ( OVX-estrogen) and ovariectomized group with injection of puerarin. After 3 months, TNF-a, CRP and E2 in the serum were tested. Meanwhile, the bone mineral density of the mandible and femur was measured. Results The body weight of OVX-puerarin group was signficantly higher than that of sham group (P < 0.01). Compared with OVX-estrogen group, the TNF-a, CRP and E2 serum level of OVX-puerarin group was significantly lower (respectively P < 0. 01 , P < 0. 05 , P < 0. 01 ). As to mandible and distal femur bone mineral density, there were no significant difference among OVX-puerarin group, sham group and OVX group, but the proximal femur bone mineral density of OVX-puerarin group was significantly higher than that of OVX-estrogen group (P < 0. 05 ) . Conclusion As an anti-inflammatory factor, puerarin can significantly reduce the level of TNF-a and CRP in the serum, but can't promote the level of E2 in the serum in ovariectomized rats. Puerarin has some therapeutic effect on the ovariectomy-induced osteoporosis, but can significantly increase the body weight of ovariectomized rats.%目的 观察葛根素对去卵巢大鼠股骨骨密度、颌骨骨密度和血清中肿瘤坏死因子(TNF-α)、C反应蛋白(CRP)及雌二醇(E2)水平的影响.方法 3月龄雌性SD大鼠随机分为假手术组(sham group)、单纯去势组( OVX group)、去势+雌二醇治疗组(OVX-estrogen group)和去势+葛根素治疗组(OVX-puerarin group).给药3个月后测量各组大鼠股骨和颌骨骨密度,血清中TNF-α和CRP及雌二醇(E2)水平.结果 OVX-puerarin组较sham组体重明显增加(P＜0.01);较OVX-estrogen组的TNF-α(P＜0.01)、CRP(P＜0.05)及E2(P＜0.01)水平显著下降;其下颌骨和股骨远端骨密度与OVX组、sham组之间无显著性差异,但其股骨近端骨密度较OVX-estrogen组明显增加(P＜0.05).结论 葛根素作为抗炎因子,能够显著降低TNF-α和CRP的水平,但并不会提高雌激素的水平;葛根素对卵巢切除所致的骨质疏松症有一定的治疗作用,但会明显提高机体体重.
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