Runx2通过促进MMP9的表达增强肝细胞肝癌迁移侵袭能力

摘要

Objective:To examine the expression of Runx2 in hepatocellular carcinoma (HCC) cells and its effect on the migration and invasion and to evaluate the expression of Runx2 in inducing MMP9 expression. Methods:Immunohistochemistry staining was per-formed to investigate the relation between the expression of Runx2 and MMP9. Transfection with Runx2 overexpression plasmid in HepG2 cells were conducted to induce exogenous expression of Runx2 and Runx2 knockdown plasmid in SMMC7721 cells and to inter-fere the quantity of Runx2 protein. The expression of Runx2 and MMP9 in HepG2 and SMMC7721 cells were analyzed by Western blot-ting and compared with the control. For wound healing assays, cell motility was assessed by measuring the movement of cells into a scarp, and the invasion assay was used to determine the function of invasive potential. Results:Immunohistochemistry staining results revealed the association of Runx2 expression with MMP9 expression in HCC specimen. After transfection, Runx2 expression in HepG2 cells increased with the expression of MMP9 and enhanced cell motility and invasion. In SMMC7721 cells, Runx2 and MMP9 expres-sion decreased and cell motility and invasion were inhibited. Conclusion:Runx2 might promote hepatocellular carcinoma migration and invasion by promoting MMP9 expression.%目的:研究Runx2对肝癌细胞中MMP9的表达以及其对肝癌细胞侵袭迁移能力的影响.方法:通过免疫组织化学染色法对2005年12月至2015年12月天津医科大学肿瘤医院和天津医科大学总医院189例肝细胞肝癌病例标本中Runx2和MMP9的表达进行分析;将Runx2过表达质粒和干扰质粒分别转染至肝癌细胞系HepG2和SMMC7721细胞中.使用Western blot法检测转染后HepG2和SMMC7721细胞中Runx2和MMP9的表达情况;划痕、侵袭实验检测Runx2对肝癌细胞迁移和侵袭能力的影响.结果:免疫组织化学结果显示Runx2的表达和MMP9的表达相关.在HepG2细胞中上调Runx2的表达,促进了MMP9的表达,增强了HepG2细胞迁移侵袭能力;在SMMC7721细胞中下调Runx2的表达,降低了MMP9的表达,抑制了SMMC7721细胞的迁移侵袭能力.结论:Runx2的表达可能参与促进肝细胞肝癌细胞MMP9的表达,进而促进肝细胞肝癌的迁移侵袭能力.