Purpose To evaluate the effect and mechanism of celecoxib ( a selective cyclooxygenase-2 inhibitor ) on the invasive ability of human nasopharyngeal carcinoma ( NPC ) line CNE-2Z. Methods MTT assay was used to detect the effect of celecoxib on NPC cell proliferation. After treated with different concentrations of celecoxib for 24 hours, the reconstituted basement membrane invasion assay was used for evaluating the changes of NPC cell invasion capacity. CD44v6 mRNA expression was detected by reverse transcription-polymerase chain reaction ( RT-PCR ). Western blot and immunocytochemistry were used to detect CD44v6 protein expression. Results MTT results indicated NPC cell proliferation was significantly suppressed by celecoxih in dose-dependent manner( P ＜ 0. 01 ). After treated with celecoxib for 24 hours. NPC cell invasion capacity was significantly decreased compared with control group( P ＜ 0. 05 ).RT-PCR results indicated that CD44v6 mRNA expression was significantly suppressed by celecoxib( P ＜ 0. 01 ). Western blot and immunocytochemistry results suggested that CD44v6 protein expressions were significantly down-regulated with celecoxib concentrations increased( P ＜ 0. 01 ). Conclusions Celecoxih inhihits invasion ability of NPC cell due to the down-regulation of CD44v6 expression.%目的 研究塞来昔布(COX-2选择性抑制剂)对鼻咽癌CNE-2Z细胞侵袭能力的影响及可能机制.方法 噻唑蓝(MTT)法检测塞来昔布对鼻咽癌CNE-2Z细胞增殖活性的影响.不同浓度塞来昔布作用鼻咽癌CNE-2Z细胞24 h后,通过重组人工基膜实验检测细胞侵袭力的改变;RT-PCR检测鼻咽癌细胞CD44v6 mRNA的表达情况;用Western blot和免疫细胞化学检测鼻咽癌细胞CD44v6蛋白表达变化.结果 MTT法结果显示,塞来昔布对鼻咽癌CNE-2Z细胞增殖有明显抑制作用,呈剂量依赖性关系(P<0.01).塞来昔布作用细胞24 h后,肿瘤细胞的侵袭力明显下降(P<0.05).RT-PCR结果显示,塞来昔布可以明显抑制CD44v6 mRNA的表达水平(P<0.01).Western blot和免疫组化结果显示,随着药物浓度的增加,CD44v6蛋白表达水平逐渐减少(P<0.01).结论 塞来昔布可能通过下调CD44v6表达而降低鼻咽癌CNE-2Z细胞侵袭能力.
展开▼
