A human high metastatic nasopharyngeal carcinoma cell strain S18 stably expressing Tet-Off Advanced, construction method and uses thereof are provided. Through introducing tet-off advanced gene into nasopharyngeal carcinoma cells, screening, amplifying and culturing positive clones and identifying, nasopharyngeal carcinoma cell strain having ability of regulating target gene expression induced by tetracycline is obtained. The nasopharyngeal carcinoma cell strain constructed by the method is named as Tet-Off Advanced nasopharyngeal carcinoma cell strain, and is deposited in China Center for Type Culture Collection as CCTCC C200959. The cell strain has stable and sensitive regulation effect on the expression of target genes. When researching a certain target gene, transfecting the said nasopharyngeal carcinoma cell strain with TRE-Tight plasmid comprising the target gene, screening, and identifying the positive clones obtained, nasopharyngeal carcinoma cell strain expressing the target gene induced by tetracycline is obtained, and the expression of the target gene can be timely and quantitatively regulated by simply regulating the content of tetracycline in environment.
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