In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma(HCC),total RNA was respectively extracted from three HCC cell strains with different metastatic potentials,HepG2,MHCC-97L and MHCC-97H.The first strand cDNA was synthesized by reverse transcription,which was then used as template to perform fluorescent quantitative polymerase chain reaction(FQ-PCR).The quantity of Twist gene expression was normalized by that of the housekeep-ing gene,GAPDH for each sample.ANOVA was used to estimate the relationship between Twist gene and metastasis potential of HCC.The results showed that the normalized initial cDNA concen-trations of Twist gene in HepG2,MHCC-97L and MHCC-97H were(9.45±0.25)×10-4,(1.82±0.41)×10-3,(3.06±0.62)×10-3,respectively.FQ-PCR revealed significant differences in the ex-pression level of Twist among HCC cell strains with different metastatic potentials.It was concluded that high expression level of Twist was closely associated with more aggressive behaviors of HCC.Twist provides a novel indicator for HCC metastasis.
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机译:Overexpression of a BRASSINAZOLE RESISTANT 1 homolog attenuates drought tolerance by suppressing the expression of PLETHORA-LIKE 1 in Setaria italica