miR-34a在结直肠癌中的表达及其临床意义

摘要

Purpose To investigate the expression of miR-34a in human colorectal carcinoma and its relationship with clinicopathological features. Methods The mRNA expression of miR-34a was determined by real-time quantitative PCR in 43 colorectal carcinoma tissues and para-cancerous normal tissues. Results Real-time quantitative PCR analysis revealed that miR-34a expression in colorectal carcinoma tissues was lower than those in the paraneoplastic colorectal tissues ( P ＜ 0. 05 ). There were no significant correlations between the expression of miR-34a and gender, age , tumor location, tumor size and lymph node metastases ( P ＞ 0. 05 ), but it related to depth of invasion and TNM stage ( P ＜ 0. 05 ). Conclusions Downregulation of miR-34a expression in the colorectal carcinoma suggests that miR-34a may be involved in the carcinogenesis and development of colorectal carcinoma. miR-34a may be a novel useful tumor marker and a prognostic factor for colorectal carcinoma.%目的 探讨miR-34a在结直肠癌中的表达及其与临床病理特征的关系.方法 应用实时荧光定量PCR法对43例结直肠癌及其癌旁正常组织中miR-34a进行定量检测.结果 结直肠癌组织中miR-34a表达水平明显高于癌旁正常组织,差异有统计学意义(P＞0.05);miR-34a在结直肠癌组织中的表达与患者性别、年龄、肿瘤部位、肿块大小、淋巴结转移均无相关性(P＞0.05),而与浸润深度、TNM分期密切相关(P＜0.05).结论 miR-34a在结直肠癌的发生、发展过程可能发挥重要作用,检测miR-34a有望成为结直肠癌新的肿瘤标记物或预后因子.