Method of absolute quantification of miRNA expression, especially miR-34a and/or miR-150 and use thereof in diagnostics and prognostic of B-cell malignancies

摘要

The present invention provides method for absolute quantification of expression mmiRNA preferably selected from miR-34a and / or miR-150, which in a biological sample from the patient provides miRNA expression, preferably selected from miR-34a and / or miR-150, together with the expression of endogenous control which is small nucleolar RNA or stably expressed miRNA, while determining the expression of the synthetic miRNA standards, preferably selected from miR-34a and / or miR-150, and the endogenous control of known numbers of molecules. The invention further provides the use of the method of quantification in vitro diagnosis of B-cell malignancies, prognostics B-cell malignancies and predicting treatment response of patients with B-cell malignancies, particularly CLL identify abnormalities TP53.