钙敏感受体对SGC-7901细胞内钙、细胞增殖和迁移的影响

摘要

目的：观察钙敏感受体( calcium sensing receptor, CaSR)在人胃癌SGC-7901细胞中的表达,对细胞内钙、增殖和迁移的影响。方法(1)采用Western blot及免疫荧光染色技术分析CaSR蛋白在人胃癌SGC-7901细胞的表达；采用激光共聚焦扫描技术检测CaSR活化对细胞内钙离子([ Ca2+] i )的影响；应用MTT法、流式细胞仪技术、细胞划痕实验检测CaSR活化对SGC-7901细胞增殖和迁移的影响。结果 CaSR蛋白在人胃癌SGC-7901细胞有表达；增加细胞外钙或者给予Calindol( CaSR激动剂)均可引起[ Ca2+] i、CaSR和E-cadherin的表达增加；CaSR活化对SGC-7901细胞的增殖无明显影响；细胞划痕实验结果显示细胞迁移能力下降。结论 CaSR在人胃癌SGC-7901细胞中有表达,并通过诱导E-cadherin的表达使SGC-7901细胞迁移能力降低。%Purpose To observe the functional expression of calcium sensing receptor ( CaSR) in human gastric cancer SGC-7901 cell line, the effect of CaSR on intracellular calcium, cell proliferation and migration of SCG-7901. Methods The expression and distribu-tion of CaSR were detected by Western blotting and immunofluorescence observation in SGC-7901. The intracellular concentration of free calcium ( [ Ca2+] i ) was determined by confocal laser scanning microscopy. MTT, flow cytometry and scratch test were used to an-alyze the impact of CaSR the proliferation and the migration capabilities of SGC-7901 cell. Results CaSR protein was expressed in SGC-7901. Extracellular calcium or calindol significantly increased the expression of [Ca2+]i, CaSR and E-cadherin;In addition, the migration capabilities were decreased. Conclusion CaSR is expressed in SGC-7901. The activation of CaSR induces the expression of E-cadherin, and decreases migration ability.