外周细胞淋巴瘤中TET2和DNMT3A的表达及意义

摘要

目的 探讨TET2和DNMT3A在外周T细胞淋巴瘤(peripheral T-cell lymphoma,PTCL)中的表达及其与PTCL免疫表型的关系.方法 应用CD3、CD4、CD10、BCL-6、CXCL-13、CD30、ALK免疫标记将PTCL分为血管免疫母细胞性T细胞淋巴瘤(angioimmu-noblastic Tcelll ymphoma,AITL)、外周T细胞淋巴瘤,非特指(peripheral T-cell lymphoma,not otherwise specified,PTCL-NOS)、ALK阳性的间变性大细胞淋巴瘤(ALK+anaplastic large cell lymphoma,ALK+ALCL)和ALK阴性的间变性大细胞淋巴瘤(ALK-anaplastic large cell lymphoma,ALK-ALCL)4种类型.应用免疫组化法检测PTCL组织中TET2和DNMT3A的表达.结果 89例PTCL中,AITL 36例,PTCL-NOS 26例,ALK-ALCL 18例,ALK+ ALCL 9例.在AITL组织中,TET2胞质表达和DNMT3A胞质及胞核表达的高表达率均高于PTCL-NOS和ALCL,差异有统计学意义(P均＜0.05).TET2胞质表达和DNMT3A胞质及胞核表达在AITL中均呈正相关(P＜0.05).结论 TET2和DNMT3A可以作为诊断AITL的分子标志物,为AITL的明确诊断提供新策略.%Purpose To investigate the expression of TET2 and DNMT3A in patients with peripheral T cell lymphoma (PTCL) and the relationship to immunophenotypes of PTCL.Methods Using a panel of immunohistochemical markers (CD3,CD4,CD10,BCL-6,CXCL-13,CD30,ALK),all cases of PTCLs were further divided into four groups,including angioimmunoblastic T cell lymphoma (AITL),peripheral T cell lymphoma,not otherwise specified (PTCL-NOS),anaplastic lymphoma kinase negative anaplastic large cell lymphoma (ALK-ALCL) and anaplastic lymphoma kinase positive anaplastic large cell lymphoma (ALK + ALCL).The expression of TET2 and DNMT3A in 89 cases of PTCL was detected by immunohistochemical analysis.Results 89 cases were divide into four subtypes,AITL (36/89),PTCL-NOS (26/89),ALKALCL (18/89),and ALK + ALCL (9/89).Immunohistochemistry staining revealed higher cytoplasmic expression of TET2 and DNMT3A in AITL than that of in PTCL-NOS and ALCL (P ＜ 0.05).And the nuclear expression of DNMT3A in patients with AITL was higher than that of PTCL-NOS and ALCL (P ＜ 0.05).The cytoplasmic expression of TET2 was positively related with both cytoplasmic and nuclear expression of DNMT3A in patients with AITL (P ＜ 0.05).Conclusion TET2 combined with DNMT3A could be used as markers in AITL diagnosis,which could provide new strategy for AITL diagnosis.