固相萃取-高效液相色谱法同时测定调味品中15种工业合成染料

摘要

建立了测定调味品中15种工业合成染料的固相萃取-高效液相色谱法(SPE-HPLC).样品经甲醇-水(1∶1,v/v)超声提取、SPE柱净化后用HPLC进行分析,流动相为10 mmol/L乙酸铵溶液(含1%乙酸)和乙腈.实验结果表明15种工业合成染料的分离效果良好,回收率为84.6%～114.2%,相对标准偏差为0.9%～10.3%;检出限为0.05～0.18 mg/kg.该方法操作简单,结果准确,重现性好,可用于同时测定调味品中非法添加的15种工业合成染料.%A new method was established for the determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography( SPE-HPLC ).The samples were extracted by methanol-water ( 1∶1. V/V ) and purified by a solid phase extraction column. Then, the chromatographic separation was achieved on a Luna C18 column by linear gradient elution. The mobile phase was 10 mmol/L ammonium acetate-acetonitrile ( containing 1％ acetic acid ). The results showed that the 15 industrial synthetic dyes can be separated efficiently. The recoveries of the 15 industrial synthetic dyes spiked in condiment were between 84.6％ and 114.2％ with the relative standard deviations of 0.9％- 10.3％. The limits of detection of this method was 0.05 -0.18 mg/kg for the 15 industrial synthetic dyes. The method is simple , sensitive . accurate , repeatable and can be used for simultaneous determination of the 15 illegally added industrial synthetic dyes.