草鱼鱼皮胶原蛋白与NIH3T3成纤维细胞的相容性

摘要

This study is aimed to test the cytocompatibility of fishskin acid-soluble collagen (FASC) of ctenopharyngodon idellus in vitro.The proliferation rates of NIH3T3 fibroblasts were detected using different concentrations of FASC solutions or coating gums by MTT experiment (n =6).The chemotaxis of fibroblasts towards FASC solutions were tested by chemotactic experiments of transwell (n =3).The cellular permeabilities in different concentrations of FASC coating gums were detected by invasive experiments of transwell (n =3).FASC solutions promote proliferation of NIH3T3 fibroblasts and the growth activity of the fibroblasts was dose-dependent on FASC.The proliferation rates of 16 μg/mL FASC solution in 48 and 96 h were (63.7 ± 7.9)％ and (87.3 ± 8.7)％,that showed significant differences compared with the control group (P ＜ 0.001).NIH3T3 fibroblasts grew on FASC coating gums without significant differences compared with the control group (P ＞ 0.05).The chemotatic indexes showed significant differences between the concentration gradient groups of FASC solutions and the control group(P ＜0.05),which indicated that FASC has chemotaxis to fibroblasts.Invasion experiments showed fibroblasts could pass through FASC coating gums.These experiments confirmed that FASC possesses favourable cytocompatibility with NIH3T3 fibroblasts.%体外检测草鱼鱼皮酸溶性胶原蛋白(FASC)的细胞相容性.采用MTT实验,检测不同浓度的FASC溶液或FASC涂层胶对NIH3T3成纤维细胞增殖能力的影响(n=6);利用transwell趋化实验,检测不同浓度的FASC溶液对成纤维细胞的趋化作用(n=3);利用transwell侵袭实验,检测不同浓度FASC涂层胶的细胞通透性(n=3).结果表明,FASC溶液浓度依赖性地促进NIH3T3成纤维细胞增殖,16 μg/mL的FASC溶液在48和96 h的增殖率分别为63.7％±7.9％和87.3％±8.7％,较对照组有显著性差异(P＜0.001).NIH3T3细胞能够在FASC涂层胶上生长,各实验组与对照组相比,增殖率均无显著性差异(P＞0.05).趋化实验显示,FASC溶液各浓度梯度组与阴性对照组的趋化指数均有显著性差异(P＜0.05),提示FASC对成纤维细胞具有趋化作用.侵袭实验结果显示,成纤维细胞可以通过FASC涂层胶.实验证实,FASC与NIH3T3细胞具有良好的相容性.