Effects of photodynamic treatment on contraction of collagen ga1s by cultured human dermal fibroblasts

摘要

Usually cell viability (CV) alter PDT IS observed in methods based on metabolic parameters (MTT assay) or dye exclusion tests (trypan-blue (TBT)) Although these tests are accurate in the determination of cell death, functional impairment can not be easily detected We examined the effects of PDT on human dermal fibroblasts (DF) to determine whether collagen gel contraction (CGC) can be inhibited and whether it correlates well with CV as measured by the TBT. 5-ammolevulinic acid (5-ALA) (1.5, 10 ug/ml) was added to DF cultured in dishes. 24 h later, a collagen type I solution was added and irradiation with a tunable dye laser (630 nm, 100 mW/cm2, 80 J/cm2) was performed. After 24 h, diameters of contracted gels and TBT were measured. As a result, PDT with 5-ALA inhibited CGC and affected CV as a function of dose level Consequently. CGC data allows a belter estimation of the threshold 5-ALA dose affecting the cells