褪黑素改善胰岛素抵抗3T3-L1脂肪细胞葡萄糖的摄取

摘要

目的:研究褪黑素对游离脂肪酸(FFA)诱导的胰岛素抵抗3T3-L1脂肪细胞葡萄糖摄取能力的影响.方法:"鸡尾酒"法培养诱导3T3-L1成纤维细胞分化成脂肪细胞,油红O染色鉴定脂肪细胞形态;利用棕榈酸(300μmol/L)诱导脂肪细胞的胰岛素抵抗,采用液体闪烁法检测细胞葡萄糖摄取能力;检测褪黑素对FFA处理的3T3-L1脂肪细胞的葡萄糖摄取能力的影响.结果:313-L1细胞经诱导分化成脂肪细胞,油红O染色呈圆形,脂滴呈典型的"戒环样"形态;用FFA处理上述细胞6h后,细胞的葡萄糖摄取能力明显降低;褪黑素可以促进FFA处理的3T3-L1脂肪细胞胰岛素介导的葡萄糖摄取.结论:FFA可以降低胰岛素诱导的体外培养的脂肪细胞葡萄糖摄取能力,可能是胰岛素抵抗的病因之一.褪黑素可以干预FFA的作用,增加细胞的葡萄糖摄取能力.%Purpose To investigate the effect of melatonin on the glucose uptake in insulin resistance 3T3-L1 preadipocytes induced by free fatty acids. Methods 3T3-L1 preadipocytes were incubated in adipogenic cocktail for 10 days and then adipocytes were obtained for study. Adipocytes of insulin resistance were induced by incubating with free fatty acids( FFA, palmitinic acid, 300 μmol/L), and then melatonin was added. Glucose uptake was detected in adipocytes by luminescent counter. Results Adipocytes with typical lipid droplet were harvested after incubating in adipogenic cocktail for 10 days. FFA (300 μmol/L, 6 h) decreased the glucose uptake in the adipocytes. However, the addition of melatonin into the FFA-treated adipocytes resulted in a increase of glucose uptake. Conclusion FFA inhibited the glucose uptake induced by insulin in the 3T3-L1 preadipocytes inducing insulin resistance, whereas melatonin attenuated the effect of FFA by promoting the glucose uptake of adipocytes.