板蓝根提取物对放射性肺损伤大鼠肺组织TGF-β表达水平的影响

摘要

目的：探讨板蓝根提取物对放射性肺损伤大鼠肺组织TGF-β表达的影响。方法将72只健康成年清洁级雌性Wistar大鼠随机分为空白对照组（A组）24只，单纯照射组（B组）24只，照射加中药组（C组）24只，A、B 2组以蒸馏水2 mL/200 g灌胃1周后，C组以每天2 mL/200g（1.6 g/200 g）灌胃服用板蓝根提取物1周，开始照射，照射期间，每周测体质量1次，至照射结束。分别在完成10次照射后（第30天），以及在照射开始后的第60天、120天，分别检测3组大鼠肺组织中TGF-β的表达；另外对各组大鼠进行肺组织形态学观察。结果 B组大鼠TGF-β的表达于照射第30天时达到高峰；A、C组大鼠TGF-β表达在各时间点均低于B组（P＜0.01）。空白对照组A组大鼠肺组织未见明显改变。B组大鼠肺间质肺泡腔内观察到炎性细胞，肺泡腔结构破坏、肺间质水肿，肺泡腔及肺间质内炎性出血；C组大鼠肺间质炎性细胞较少，肺泡腔破坏不明显，无肺间质水肿，也无炎性出血症状发生，B、C2组间比较不明显。结论板蓝根提取物可减少放射性损伤大鼠肺成纤维细胞TGF-β表达，可减少放射性肺损伤的功效。具有保护肺组织不受损害，抑制肺泡通透性，预防肺水肿发生的作用。%Objective To investigate the effect of extraction of radix isatidis on the expression of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury.Methods 72 female Wistar rats were randomly divided into 3 groups,blank control group(groupA,n=24),simple irradiation group(group B,n=24),irradiation with Chinese medicine group(group C,n=24).Rats in group A and group B were given distilled water 2 mL/200g by gavage for one week,the rats in group C were given extraction of radix isatidis 2 mL/200g(1.6 g/200 g)per day for one week,then the rats received irradiation,during irradiation,the rats' weight was measured once a week,to illuminate the end.After 10-time irradiation(the 30th day), and at 60 days and at 120 days after irradiation,the expression levels of TGF-βin lung tissue were detected;In addition each rat lung tissue morphology was observed.Results The expression levels of TGF-βprotein in group B reached peak at the 30 days after irradiation,which at different time points in group A and group C was significantly lower than that in group B(P<0.01).Rats of group A had no significant change in lung tissue.Rats of group B were observed inflammatory cells in interstitial pulmonary alveolar,alveolar structural damaging,interstitial pulmonary edema,interstitial hemorrhage and inflammatory pulmonary with alveolar cavity;Rats of group C pulmonary interstitial fewer inflammatory cells in alveolar damage were not obvious, with no-pulmonary interstitial edema, hemorrhage and no inflammatory symptoms, comparison between group B and C was not obvious. Conclusion Extraction of radix isatidis could reduce the expression levels of transforming growth factor(TGF-β)in lung tissues of rats with radioactive lung injury ,has better preventive effect on radioactive lung injury,could protect lungtissue from damaging,inhibite alveolar permeability,and present the effect of pulmonary edema.