TGF-β在放射性肺损伤大鼠肺组织中的表达及意义

摘要

Objective To investigate the expression of TGB-β on lung tissue of rats with radiation induced lung injury. Methods Thirty-five male Wistar rats were randomly divided into 7 groups (n = 5) :control group and treatment group (received irradiation on the the right hemithorax or sham right lung irradiation with single 30Gy, lday, 1,2,4,8 ,and 12 weeks). The rats were sacrificed at 1 day ,1,2,4,8, and 12 weeks after the irradiation, and lung tissues were separated. The expression of TGF-β was detected by immuno-histochemistry. Results The expression of TGF-β in fibroblast (FB) significantly increased after irradiation, compared with control group (P = 0. 000). The degree of injury increased with the time passing. Conclusion TGF-β was an important biological mediator during the activation of fibroblast. We suspected that FB might be the target cell for prevention and cure the radiation induced lung injury.%目的 探讨转化生长因子-β(TGF-β)在放射性肺损伤大鼠肺组织中的表达及意义.方法 选择无特定病原体(SPF)级健康Wistar大鼠35只,随机分为对照组(5只)、照射组(30只,1 d,1、2、4、8、12周各5只).在照射1 d,1、2、4、8、12周获取标本,采用免疫组化法观察放射性肺损伤大鼠肺组织细胞TGF-β水平变化.结果 照射组TGF-β在肺成纤维细胞中的表达高于对照组,差异有统计学意义(P=0.000),其损伤程度随时间的推移而增加.结论 TGF-β是肺成纤维细胞(FB)活化的最重要生物介质之一,阻断TGF-β通路及FB的活化,可能为减少照射后肺间质炎症和纤维化的程度带来新的希望.