ABSTRACT The present invention related to a novel extract for controlling hyperglycaemia and hyperlipidaemia in the management of diabetes. Aim of the study: To determine the effectiveness of unripe fruits of Bryonopsis laciniosa (BL) extracts as an antidiabetic agent in vitro and in vivo. Preparation of Extract: Methanolic extract of BL and its fractions of Bryonopsis laciniosa Method: Hypoglycemic effect in normal and antihyperglycemic activityin diabetic rats, rat hemi-diaphragm,cell line(3T3-Ll cells and RIN cells), Rat hemi diaphragm method. Results: The extract increased glucose uptake in 3T3-L1 cells and in rat hemi-diaphragm extract increased basal and glucose-induced insulin secretion in RINm cells. They have potential antihyperglycaemic , antihyperlipidaemic effects, No toxic effect was observed after 28 days treatment, Ethyl acetate fraction of BL (EAFBL) exhibited a concentration dependent free radical scavenging activity. In sub acute study in streptozotocin induced diabetic rats, EAFBL showed antioxidant activity by significantly decreasing the level of lipidperoxidative marker, malondialdehyde (MDA), a protective action against cell damage required in diabetes therapy. Conclusions: Bryonopsis laciniosa extract has effects that enhance glucose uptake and provide the basis for antidiabetic activity of the extract.
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