肾上腺皮质激素对脊髓炎患者血清NMO-IgG抗体检测的影响

摘要

Objective To evaluate the effect of adrenocortical hormone on detection of NMO-IgG in neuromyelitis optica ( NMO ) patients.Methods 30 cases with NMO collected in the hospital according to the figures were randomly divided into adrenocorticotropic hormone group and control group, 15 cases in each group.adrenocorticotropic hormone group were given glucocorticoid therapy on the basis of conventional therapy.The control group were treated with conventional therapy, and The serum NMO-IgG levels were detected by indirect immunofluorescence taking stable expression of human origin aquaporin -4 (AQP4) in human embryonic kidney 293 (HEK293) cell line as substrate.Results 19 cases (63.3%) serum NMO-IgG positive patients in 30 cases with spinal cord inflammation, serum antibody titers from 1:115 to 1砄6355.The positive rate of serum NMO-IgG in adrenocorticotropic hormone group was 86.7%, which was higher than 40.0% in control group (χ2 =7.03,P<0.05).Conclusion The adrenocortical hormone could help the detection of NMO-IgG antibody and increase the efficiency of clinical diagnosis.%目的：肾上腺皮质激素对视神经脊髓炎（ neuromyelitis optica，NMO）患者血清中NMO-IgG抗体检测研究影响。方法收集武汉市汉口医院中临床诊断为视神经脊髓炎的患者30例，按随机数字表法分为肾上腺皮质激素组和对照组，每组15例。肾上腺皮质激素组患者在常规治疗的基础上给予肾上腺皮质激素治疗，对照组患者给予常规治疗。底物为稳定表达人源水通道蛋白-4（aquaporin-4，AQP4）的人胚肾293（human embryonic kidney 293，HEK293）细胞株，患者血清NMO-IgG水平的检测采用间接免疫荧光法。结果30例视神经脊髓炎患者血清NMO-IgG阳性19例（63.3％），血清抗体滴度为1砄115～1砄6355。肾上腺皮质激素组视神经脊髓炎患者血清NMO-IgG阳性率（86.7％）高于对照组患者（40.0％）（χ2＝7.03，P＜0.05）。结论肾上腺皮质激素有助于NMO-IgG抗体的检测，可以提高临床诊断率。