Hepatitis C Virus Genotyping and Serum Anti-F Antibody Detection of HCV Patients in the South of Jiangsu Province of China

摘要

This study investigated the distribution of hepatitis C virus (HCV) genotypes and assessed the prevalence and distribution of serum anti-F in patients with hepatitis C virus (HCV) infection in the south of Jiangsu province of China. One hundred and fifty-eight anti-HCV positive sera samples were indentified by the PCR with type specific primers designed according to the sequence of 5’-non-coding region (5’ NCR). Indirect ELISA was carried to detect anti-F in sera of 120 HCV patients and 10 donors. Ninty-five HCV RNA positive patients (95/158) were classified as 80 (80/95, 84.2%) genotype 1b, 5 (5/95, 5.3%) genotype 2, 5 (5/95, 5.3%) genotype 1b/2 mixed and 5 (5/95, 5.3%) genotype undetermined. Then genotype 1b was the predominant in the south of jiangsu province of China. The difference on the distribution of HCV genotypes was significant between female and male patients（p<0.05）, but not in different kinds of HCV patients. Eighty-two samples (68%) out of the 120 HCV infected patients exhibited a positive anti-F reaction, showing significant difference from the controls without HCV infection (p<0.01). Chi-square test showed that there were no significant correlations between anti-F antibody and sex, HCV RNA serum , types of HCV RNA. Patients of midrange, severe range and hepatic cirrhosis have higher rate than others（p<0.05）, the positive rate of anti-F is higher in patients over fifties（p<0.01）. This report of the serum anti-F prevalence and distribution of anti-F in the South of Jiangsu Province hepatitis C patients may help the investigation of the pathogenesis of HCV, and functions of F protein.