翻白草对大鼠溃疡性结肠炎的药效及作用机制研究

摘要

Objects:To study the pesticide effectiveness of Potentilla discolor Bunge on treating ulcerative colitis(UC) rats,and to explore the molecular mechanism of Potentilla discolor Bunge in the treatment of UC.Methods:The UC rats were induced by trinitrobenzene sulfonic acid-ethanol enema.The rats were divided into six groups:blank control group,model control group,sulfasalazine group,low potentilla discolor,Middle and high dose groups.After 14 consecutive days,chloral hydrate was used to make the rats narcotized and colonic tissue was embedded in paraffin,serially cut and stained for microscopic observation.TLR4 and NF-κBP65 in the remaining colon tissue were detected by western blot.Results:Compared with the blank control group,the DAI index and pathological morphologic change in the model group were significantly different.The therapeutic effect in low-dose group was the best.Compared with the blank control group,the expression of NF-κBP65 was significantly increased in the model group.All the Potentilla discolor Bunge groups could down regulate the NF-κBP65 expression level.Conclusion:Potentilla discolor Bunge could exert its effect on UC by regulating the expression level of NF-κBP65 in UC rats.%目的:研究翻白草对大鼠溃疡性结肠炎的药效作用,并探讨翻白草治疗UC的可能分子机制.方法:采用三硝基苯磺酸-乙醇灌肠法复制溃疡性结肠炎(UC)大鼠模型,实验分为空白对照组、模型对照组、柳氮磺吡啶组、翻白草低、中、高剂量组6组.连续给药14 d后水合氯醛麻醉,取病变结肠组织石蜡包埋、切片、染色后镜下观察,剩余结肠组织采用western blot方法分别检测TLR4及NF-κBP65.结果:DAI指数及病理形态变化表明,与空白组比较模型组差异有统计学意义,翻白草低剂量组治疗效果最佳;与空白组比较模型组大鼠结肠组织NF-κBP65表达水平显著增加,翻白草给药组均能下调NF-κBP65的表达水平.结论:翻白草能通过调控NF-κBP65表达水平以发挥对UC的治愈作用.