Molecular imprinting technology was employed to produce one kind of rhodamine B ( RhB ) molecularly imprinted polymer (MIP) microspheres by precipitation polymerization using RhB, acrylamide, ethylene glycol dimethacrylatea ( EGDMA ) , azobisisobutyronitrile ( AIBN ) , and acetonitrile as template, functional monomer, cross-linking agent, initiator and porogen, respectively. The resultant microspheres were characterized by scanning electron microscopy. The binding constant and chemical combination ratio of the complex formed between the template and the functional monomer were obtained by UV spectrophotometry, which showed that the RhB-monomer forms 1∶1 complexes and the binding constant is 5. 3 × 103 L/mol. The effects of types and volumes of the porogen on the size and the uniformity of microspheres were also discussed. After packing the microspheres into column, it was used to absorb RhB from red pepper. The extraction conditions of molecular imprinting solid phase extraction ( MISPE) column for RhB were optimized. Pepper samples spiked with RhB were extracted by MISPE column and analyzed by high performance liquid chromatography. The recoveries of MISPE column for RhB extraction were found to be 91.7％～ 103.5％ , indicating the feasibility of the prepared MIPs for RhB extraction.%以罗丹明B为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备了罗丹明B分子印迹聚合物(MIP)微球,并用扫描电子显微镜表征.采用紫外分光光度法测定了印迹分子罗丹明B与功能单体丙烯酰胺二者之间的结合常数(K=5.303 × 10(3)(mol/L)(-1))和化学计量比(n=1).考察了沉淀剂的种类和用量对聚合物微球的影响.将分子印迹聚合物微球应用于固相萃取材料自制固相萃取柱,从加标罗丹明B的红椒粉中萃取罗丹明B.本文优化了固相萃取条件,高效液相色谱检测表明,在一定的萃取条件下,分子印迹聚合物对加标量为0.479 mg/kg的辣椒中罗丹明B的萃取加标回收率可达91.7%～103.5%.
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