鼠伤寒沙门氏菌粘附素样糖蛋白的分离与鉴定

摘要

鼠伤寒沙门氏菌用去污剂NP-40处理，获得细菌外膜增溶液，经ConA-Sepharose 4B亲和层析，甘露糖洗脱，得到了ConA结合糖蛋白，SDS-PAGE电泳显示出了5条蛋白带，分子量在15～43 kD之间。用不同处理并借助扫描电镜观察细菌在雏鸡肠上皮的粘附情况，表明分离的ConA结合糖蛋白具有粘附素样作用，肠粘膜表面含乙酰氨基葡萄糖的糖复合物则可能是粘附素的受体。%Salmonella typhimurium was solubilized by 2％ NP-40．The extract was absorbed to Con A-Sepharose 4B column and eluted by D（＋）-Mannose．The obtained Con A-binding glycoprotein （CBG） was subjected to SDS-PAGE and showed five bands with molecular weights between 15～43 kDa．The role of CBG in the adherence of bacteria to intestinal epithelium was investigated．The CBG solution （containing 0．25 μg CBG）was injected to the ligated ileum of two-day-old chicks and incubated for 15 min．1×108 bacteria were injected into the ileum and incubated for 15 min．By scanning electron microscopy，it was found that the adherence of bacteria to the intestinal epithelium was significantly inhibited by CBG．This result suggests that the CBG might be adhesin-like component on the surface of Salmonella typhimurium．