The purpose of this study was to analyze MSTN and p21 mRNA expression profiles and differences among chicken, quail and hybrids of chicken-quail during embryonic myogenesis, and evaluate the potential relationships between their relative expression and myogenesis. The cross-bred eggs of quail ♀ were acquired by artificial insemination with chicken( ♂ )-semen, the eggs of chicken, quail and hybrid were hatched in one batch according to the standard condition of chicken, the breast muscle tissues was collected from day 7 to 17. The expression of MSTN and p21 mRNA in different species every day was revealed by real-time quantitative PCR. The expression of MSTN and p21 mRNA was detected from day 7 to 17. Peak of MSTN and p2l expression was noticed at day 9 in chicken, and day 7 in quail, with the development of muscle, the expression level was rising, which was almost maintained at this level until day 17. And the peak expression was also noticed at day 9 in hybrid which was in accordance with chicken, however, the expression of p21 mRNA in hybrid was significantly higher than that in chicken and quail (P<0. 01). The expression profile of MSTN was in accordance with the profile of the withdrawal of myoblasts from the cell cycle, and MSTN could up-regulated the expression of p2l specifically during embryonic myogenesis.%旨在研究MSTN和p21基因在鸡、鹌鹑和杂交禽胚胎肌肉发育过程中的表达规律,比较这2个基因在杂交禽与亲本鸡和鹌鹑中的差异表达,探讨MSTN和p21基因与肌肉发育的关系.通过人工受精获得鸡(♂)与鹌鹑(♀)杂交禽蛋,与鸡蛋、鹌鹁蛋按照鸡标准孵化条件同批入孵,采集第7～17天活胚胸肌组织,应用实时荧光定量PCR技术检测MSTN和p21基因在3个物种中每一天mRNA的相对表达量.MSTN和p21基因在胚胎肌肉发育的第7～17天均有表达,这2个基因mRNA的表达在鸡胚中第9天到达第1次峰值,在鹌鹑中第7天到达第1次峰值,后都随胚胎的发育表达水平上升,并维持相对稳定的高水平表达.杂交禽的表达规律与鸡一致,也在第9天达到峰值,而P21 mRNA表达极显著高于鸡和鹌鹑(P＜0.01).MSTN mRNA表达规律与成肌细胞退出细胞周期的时间规律一致；在胚胎肌肉发育过程中,MSTN基因特异性上调p21的表达.
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