广西某市屠宰猪淋巴结猪圆环病毒3型的病原检测及遗传演化分析

摘要

In order to understand the epidemic status and phylogenetic relationship of porcine circo-virus type 3 in healthy slaughter pigs,181 lymph nodes collected from slaughterhouse in Guangxi were detected for PCV3 pathogens.PCV3 infection in healthy slaughter pigs was detected by PCR method.The positive rate of PCV3 was 24.31％,which suggest that the infection of PCV3 in healthy pigs is more severe.Six PCV3 complete genome sequences were amplified with PCR in the positive samples.Compared with PCV3 complete genome in GenBank and the results showed the nucleotide homology of PCV3 sequences was 98.6％-99.9％and genes of PCV3 were very conservative and similar.Genetic evolution analysis of PCV3 ORF2 gene showed that PCV3 strains could be divided into two major types.Analyses of the phylogenetic relationship of PCVs Cap and suggesting cross protection between PCV2 and PCV3 is impossible.%为了解猪圆环病毒3型(PCV3)在广西地区健康猪群中的遗传变异情况,对广西某市屠宰场健康猪的181份淋巴结进行PCV3的病原检测,结果PCV3阳性率为24.31％.这说明在健康屠宰猪群中PCV3的感染率较高,带毒情况较为严重.对其中的阳性样品进行全序列扩增,最终得到6条PCV3全基因组序列.将试验所得 PCV3全基因组序列与GenBank上已发表的国内外毒株全基因组序列相比较,结果发现PCV3全基因组相似性为98.6％～99.9％,变异程度低,较为保守;对PCV3 ORF2基因进行遗传演化分析,发现 PCV3毒株可分为两大型.通过对PCVs(PCV1 、PCV2和PCV3)Cap蛋白相似性进行比对分析,推测 PCV2与PCV3不具有交叉保护性.