基于荧光共振能量转移的r金纳米粒子/碳量子点荧光纳米探针检测精氨酸

摘要

利用碳量子点(CQDs)和金纳米粒子(AuNPs)间的荧光共振能量转移(FRET)效应成功实现了精氨酸的快速检测.采用一步微波辅助法合成具有优良荧光性能的碳量子点(CQDs),并对AuNPs/CQDs复合材料进行相应表征,分析了其猝灭和检测机制.对AuNPs/CQDs的添加量、pH值和反应时间进行了优化.在优化条件下,将此荧光体系用于精氨酸含量的检测,结果表明,荧光强度和精氨酸浓度在0.1～10.0μmol/L范围内呈良好的线性关系(R2=0.993),检出限为5.8 nmol/L.采用本方法测定了葡萄汁中精氨酸的含量,回收率为105.4％ ～110.8％,表明本方法可用于果汁中精氨酸的实际检测.%A novel method for rapid detection of arginine based on fluorescence resonance energy transfer effect (FRET) between carbon quantum dots ( CQDs) and gold nanoparticles ( AuNPs) was developed. Firstly, the CQDs with excellent fluorescence properties were synthesized by one-step microwave assisted method. The AuNPs/ CQDs composites were characterized and their quenching mechanism was analyzed. Then the amount of AuNPs/ CQDs, the pH value and the reaction time were optimal. Under the optimum conditions, the fluorescence system was used to detect the content of arginine, showing a good linear relationship ( R2 = 0. 993 ) between fluorescence intensity and concentration of arginine in the range of 0. 1-10. 0 μmol/ L, and the detection limit was 5. 8 nmol/ L. Finally, the content of arginine in grape juice was determined by this method with recoveries of 105. 4％ -110. 8％ , which indicated that the proposed FRET system had the potential for practical detection of arginine in fruit juice.