男性免疫性不育患者外周血T细胞亚型分布

摘要

目的：分析男性免疫性不育患者外周血T细胞及Th细胞亚型分布情况，揭示男性免疫性不育者的机体免疫状态。方法采用流式细胞术检测男性免疫性不育患者外周血T细胞亚型分布，采用实时荧光定量PCR检测Th1和Th2的效应因子γ干扰素（interferon-γ， IFN-γ）和白细胞介素-4（Interleukin-4， IL-4）在基因转录水平的表达。结果流式细胞术检测结果显示，与正常对照相比，免疫性不育患者CD3＋T细胞占淋巴细胞百分比明显升高， CD8＋T细胞占淋巴细胞百分比明显升高， CD4＋T细胞占淋巴细胞百分比明显降低，免疫性不育者 CD4／CD8比值下降并倒置，差异均具有统计学意义（P＜0.05）。 RT-PCR检测结果显示，与正常对照相比， IFN-γmRNA在免疫性不育组的表达量显著上调， IL-4 mRNA的表达量显著下调，差异均具有统计学意义（P＜0.05）。结论男性免疫性不育患者免疫系统出现T淋巴细胞功能失调及Th细胞分化向Th1细胞方向极化偏移。%Objective To analyze the distribution of T cells and Th cells in the peripheral blood of male immune-related infertility patients and to reveal the immune state of male immune-related infertility. Methods Flow cytometry was used to detect the distribution of T cell subsets in the peripheral blood of male immune-related infertility patients.Real-time fluorescence quantitative PCR ( RT-PCR) was used to detect the expression of IFN-γand IL-4, which were effector of Th1 and Th2 at the gene transcription level.Results The results of flow cytometry showed that the percentage of CD3 +T cells and CD8 +T cells in lymphocytes in immune-related infertility patients was significantly higher than that of normal controls.The percentage of CD4 +T cells in lymphocytes was significantly decreased, and the ratio of CD4/CD8 cells in immune-related infertility was decreased and inversion.The difference was statistically significant ( P<0.05 ) .RT-PCR test results showed that the expression of IFN-γmRNA in immune-related infertility group was significantly up-regulated, and the expression of IL-4 mRNA was significantly lower than that of normal control.The difference was statistically significant ( P <0.05 ) .Conclusion The immune system of male immune-related infertility has prominent T lymphocyte dysfunction and Th cell differentiation is shifted to Th1 direction and Th1 cell polarization.