日本血吸虫重叠 HBV 感染小鼠模型的建立

摘要

Objective To establish a mouse model of Schistosoma japonicum and hepatitis B virus (HBV)co-infection.Methods Female BAL B/cJ mice of 6-8 weeks old were infected by cercariae of Schistosoma japonicumat.In 8 weeks,HBV replication competent plasmid of genotype B was injected to these mice through high-pressure tail intravenous. Then Blood samples were collected at day 3 and 7,respectively.Levels of HBsAg,HBeAg,ALT,HA,and HBV titer were detected. In addition, HBcAg expression in hepatocyte and formation of egg-granuloma were examined by immunohistochemical staining and HE staining,respectively.Related biological indexes were compared between different groups.Results HBcAg expresssion was detected in hepatocytes around ova granuloma in liver tissue of super-infection mice.HBsAg,HBeAg and progeny virus were also detected in mice serum.ALT level in super-infection mice was lower than that of merely HBV-infected mice,but higher than that of group infected with Schistosoma japonicum alone.While HA level was significantly higher than that of mice infected by HBV or Schistosoma japonicum,respectively.Conclusion The mice model with super-infection of Schistosoma japonicum and HBV was constructed successfully.It could provide an experimental platform of small animal for subsequent research associated with Schistosoma japonicum and HBV superinfection.%目的：建立日本血吸虫重叠 HBV 感染小鼠模型，观察其生物学表现。方法日本血吸虫尾蚴感染6～8周龄雌性 BALB／cJ 小鼠，8周后，通过高压尾静脉注射 B 基因型 HBV 可复制性克隆，分别在注射后第3、7天，检测血清HBV DNA、HBsAg、HBeAg、ALT、透明质酸（HA）水平；检测 HBcAg 在肝细胞内表达情况和虫卵肉芽肿形成情况。比较重叠感染相关生物学指标和单独感染组的不同。结果重叠感染组小鼠肝组织内可见 HBcAg 在虫卵肉芽肿周围肝细胞内表达；可以正常分泌 HBsAg 和 HBeAg 以及子代病毒；其血清 ALT 水平低于 HBV 感染组，高于血吸虫感染组；HA 含量相对明显升高。结论成功建立了日本血吸虫重叠 HBV 感染小鼠模型，为后续研究此类重叠感染提供了良好的小动物实验平台。