肝组织HBV cccDNA转录相关因子表达分析

摘要

Objective To investigate the expression and significance of SWI/SNF-related matrix-associated actindependent regulator of chromatin subfamily B member 1 (SMARCB1),interferon stimulated exonuclease gene 20 (ISG20) and P300/CBP-associated factor (PCAF) in the liver tissues from different stages of chronic hepatitis B (CHB).Methods The expression of SMARCB1,ISG20 and PCAF in liver tissues were detected using immunohistochemistry.All the 32 patients were divided into 3 groups,including 11 cases of immune tolerance (IT),13 cases of immune clearance (IC) and 8 cases of low replication stage (LR).The correlations among the expression of SMARCB1,ISG20 and PCAF at different stages of CHB and the quantification of hepatitis B surface antigen (HBsAg) were analyzed,respectively.Results Expressions of SMARCB1,ISG20 and PCAF varied at different stages of CHB,respectively.SMARCB1 expression in the CHB patients from high to low was IT patients,IC patients and LR patients.The expression of ISG20 was higher in IC patients than that in IT and LR patients.PCAF had the highest expression in IT patients.The expressions of SMARCB1 and PCAF were positively correlated with HBsAg,while the expression of ISG20 was negatively correlated with HBsAg.Conclusion The expressions of SMARCB1,ISG20 and PCAF were closely related to cccDNA transcriptional activity,which might help to evaluate cccDNA transcription activity comprehensively from viral factors and host immune factors.%目的 探讨前期细胞模型研究中筛选到的与cccDNA转录活性相关的宿主分子SMARCB1、ISG20及PCAF在慢性乙型肝炎(CHB)不同阶段肝组织中的表达及意义.方法 应用免疫组化法检测32例CHB患者肝组织SMARCB1、ISG20及PCAF的表达情况,患者中免疫耐受期(IT) 11例,免疫清除期(IC)13例,低复制期(LR)8例.针对SMARCB1、ISG20及PCAF在CHB不同阶段的表达情况及其与HBsAg定量之间的相关性进行分析.结果 CHB不同阶段中肝组织SMARCB1、ISG20及PCAF的表达不同.其中SMARCB1以IT患者中表达含量最高,IC患者其次,LR患者最低;ISG20表达在IC患者中最高,IT及LR患者中均较低;PCAF表达在IT患者中最高.SMARCB1、PCAF的表达与HBsAg呈正相关性,ISG20的表达与HBsAg呈负相关性.结论 SMARCB1、ISG20及PCAF的表达与cccDNA转录活性密切相关,有助于从病毒因素及宿主免疫因素等综合评估cccDNA转录活性.