ELISA法抗-HCV与荧光定量PCR HCV-RNA检测结果对比分析

摘要

目的：探讨丙型肝炎酶联免疫吸附试验(ELISA)法和荧光定量 PCR HCV-RNA 检测血清中抗-HCV 与HCV-RNA的结果。方法：采集102例慢性丙肝患者的血清，用ELISA法和荧光定量PCR HCV-RNA法进行检测。结果：102例患者中，抗-HCV(+)组74例，检出率72.5%(74/102)，抗-HCV(-)组28例，检出率27.5%(28/102)，在HCV-RNA检测，74例中54例病毒含量≥104 IU/mL，检出率73.0%(54/74)，28例中3例病毒含量≥104 IU/mL，检出率10.7%(3/28)，两组比较，差异有统计学意义(P＜0.05)。结论：联合检测抗-HCV与HCV-RNA可提高丙肝患者的检出率，通过测定结果的对比，抗-HCV诊断丙肝患者有漏诊现象，荧光定量PCR HCV-RNA的特异性高，敏感度强，是临床诊断丙型肝炎的重要依据。%Objective:To explore the results of ELISA method and fluorescence quantitative PCR HCV-RNA for detection of anti-HCV and HCV-RNA in serum in hepatitis C.Methods:Serum of 102 patients with chronic hepatitis C was collected.They were detected by ELISA method and fluorescence quantitative PCR HCV-RNA.Results:In 102 patients,74 cases were in anti-HCV(+) group,28 cases were in anti-HCV(-) group,and the detection rate was 27.5%(28/102).In HCV-RNA detection,in 74 cases,the virus content of 54 cases were more than 104 IU/mL,and the detection rate was 73%(54/74);in 28 cases,the virus content of 3 cases were more than 104 IU/mL,and the detection rate was 10.7%(3/28);there was significant difference between groups(P<0.05).Conclusion:The combined detection of anti-HCV and HCV-RNA can improve the detection rate of hepatitis C patients.By comparing the results of the test results,anti-HCV had misdiagnosis phenomenon in the diagnosis of hepatitis C patients,in fluorescence quantitative PCR HCV-RNA,the specificity was high,and sensitivity was strong.It was an important basis for clinical diagnosis of hepatitis C.